effect of protein and dna components of toxoplasma gondii on il-12 production from dendritic cells and t cell proliferation

objective: the aim of the present study was to investigate the effect of protein and dna components of toxoplasma gondii on maturation of dendritic cells and their efficiency in il-12 production and proliferation of t cells. materials and methods: for dc generation, bone marrow cells were cultured in the presence of gm-csf and il-4 for 5 days. tumor lysate and protein or dna components of toxoplasma gondii were added to the culture media and incubated for another 2 days. lps was added as control for dc maturation. proliferation of t cells were determined by mlr and il-12 production was measured by elisa kit. maturation of dendritic cell were determined by flowcytometry. results: dcs treatment with protein components of toxoplasma gondii caused a significant increase in il-12 production and proliferation of t cells (p